research

Research 

1. Vaccine research and development
  1. (1) Oral vaccine
  2. (2) Nasal vaccine
  3. (3) Recombinant BCG vaccine
2.Immunotherapy for allergic and autoimmune diseases
  1. (1) Allergic asthma
  2. (2) Autoimmune myocarditis
  3. (3) Ulcerative colitis

1. Vaccine research and development 

   We are investigating the various types of novel vaccines by using new advanced technology.

(1) Oral vaccine


Oral vaccine figure
   We are investigating whether virus-like particles (VLP) composed from open reading frame 2 (ORF2) of hepatitis E virus (HEV), which is an oral transmissible virus, can be utilized as carrier molecules for an irrelevant epitope, such as HIV or influenza virus, and for stimulating mucosal immunity by oral administration. Oral administration of DNA vaccines using VLP of HEV is also examined. DNA vaccine (tuberculosis, cytokines or SOCS DNA vaccine) are encapsulated into VLP, and assessed therapeutic effects in not only infectious diseases but also allergy and autoimmune diseases.

Che-Yen Wang, Naoyuki Miyazaki, Tetsuo Yamashita, Akifumi Higashiura, Atsushi Nakagawa, Tian-Cheng Li, Naokazu Takeda, Li Xing, Erik Hjalmarsson, Claes Friberg, Der-Ming Liou, Yen-Jen Sung, Tomitake Tsukihara, Yoshiharu Matsuura, Tatsuo Miyamura and R. Holland Cheng
Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle
Acta Cryst. (2008). F64, 318-322






(2) Nasal vaccine


   Human parainfluenza type 2 virus (hPIV2) is one of the human respiratory infectious viruses, and this virus can be derived by reverse genetics technology. We are investigating whether this virus can be used as a viral vector for vaccine and gene therapy.

Nasal vaccine figure




(3) Recombinant BCG vaccine


   A BCG vector is advantageous over other bacterial vectors. We are investigating the ability of recombinant BCG having various epitopes as a novel vaccine for prevention of infection or establishment of autoimmune disease. This project is in collaboration with Japan BCG Laboratory (Dr. Kazuhiro Matsuo).

BCG vaccine figure

2 . Immunotherapy for allergic and autoimmune diseases 

   We are investigating the therapeutic effects of immunotherapy with Ag85B, which is an immunogenic protein of Mycobacteria, cytokines and their antagonists using hPIV2 vector or DNA vaccine.

(1) Allergic asthma


   We are assessing the therapeutic effects of Ag85B and cytokine DNA vaccines in an allergic asthma model, and the recombinant proteins of Ag85B are also made as a new anti-allergy drug.

Allergic asthma Photo





(2) Autoimmune myocarditis.

Autoimmune myocarditis Photo
   To elucidate the immunological mechanisms of myocarditis, we are trying to establish novel animal models of autoimmune myocarditis by using the various vaccine vectors encoding cDNA of myosin. This project is being conducted in collaboration with the Department of Internal Medicine in Tsukuba University of Medicine (Prof. Kazutaka Aonuma).






(3) Ulcerative colitis


   We are assessing the therapeutic effects of oral vaccine using VLP in ulcerative colitis models.